techniques used in cell biology MCQs

1. Isolation of cellular components is called:

A. Tissue culture

B. Cell fractionation

C. Electrophoresis

D. Spectrophotometry

2. Cell fractionation separates organelles on the basis of:

A. Shape only

B. Color

C. Size and density

D. Temperature

3. First step of cell fractionation is:

A. Homogenization

B. Sedimentation

C. Staining

D. Filtration

4. Enzyme used to digest middle lamella in plant cells:

A. Amylase

B. Protease

C. Lipase

D. Pectinase

5. The process of settling organelles by centrifugation is called:

A. Homogenization

B. Sedimentation

C. Chromatography

D. Micrometry

6. Heaviest organelles settle at:

A. Low speed centrifugation

B. High speed centrifugation

C. Ultrafiltration

D. Electrophoresis

7. Pellet rich in mitochondria is obtained at:

A. 800g

B. 150,000g

C. 100,000g

D. 500g

8. Differential staining is used to:

A. Measure size

B. Distinguish structures

C. Culture cells

D. Separate proteins

9. Gram staining is an example of:

A. Simple staining

B. Vital staining

C. Negative staining

D. Differential staining

10. Microdissection uses:

A. Microscope

B. Centrifuge

C. Spectrophotometer

D. Incubator

11. Tissue culture is also called:

A. Sedimentation

B. Fractionation

C. Cloning

D. Staining

12. Plant cells are totipotent because:

A. They divide slowly

B. Each has full genetic potential

C. They lack nucleus

D. They are haploid

13. F.C. Steward regenerated plant from:

A. Phloem tissue

B. Xylem

C. Leaf

D. Root tip

14. Callus is:

A. Differentiated tissue

B. Dead tissue

C. Undifferentiated cells

D. Vascular tissue

15. Chromatography separates components based on:

A. Color only

B. Weight only

C. Density

D. Rate of movement

16. Stationary phase in paper chromatography is:

A. Solvent

B. Filter paper

C. Ink

D. Water

17. Electrophoresis separates molecules based on:

A. Color

B. Density

C. Charge and size

D. Shape

18. DNA moves towards:

A. Cathode

B. Anode

C. Neutral zone

D. Buffer

19. Smaller molecules in electrophoresis:

A. Move faster

B. Move slower

C. Do not move

D. Move randomly

20. Instrument used in spectrophotometry:

A. Microscope

B. Centrifuge

C. Electrophoresis unit

D. Spectrophotometer

21. Spectrophotometry measures:

A. Cell size

B. Light absorption

C. Electric charge

D. Density

22. Magnification × resolution gives:

A. Size

B. Contrast

C. Clarity

D. Color

23. Resolution refers to:

A. Distinguishing close objects

B. Size increase

C. Darkness

D. Color contrast

24. Electron microscope uses:

A. Light rays

B. UV rays

C. X-rays

D. Electron beams

25. Phase contrast microscope is used for:

A. Stained cells

B. Transparent cells

C. Dead cells

D. Fossils

26. Micrometry deals with:

A. Cell division

B. Cell culture

C. Measurement

D. Staining

27. Ocular micrometer has:

A. Absolute scale

B. Centimeters

C. Inches

D. No absolute value

28. Stage micrometer is used for:

A. Calibration

B. Magnification

C. Contrast

D. Resolution

29. Unit of micrometer is:

A. mm

B. µm

C. cm

D. nm

30. 1 µm equals:

A. 1 mm

B. 0.01 mm

C. 0.001 mm

D. 0.1 mm